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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr.
Catalog #	0014-03
Applications	Suitable for use in Western Blot. No recommended for use in Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Western Blot: 1ug/ml detected 14-3-3 protein in a RIPA lysate prepared from human A431 epidermoid (A0001-50), mouse 3T3 fibroblast, rat L6 skeletal fibroblast, chinese hamster ovary cells and rabbit brain cytosol.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
	I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
	I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Included Positive Control	A0001-50: Non-stimulated A431 cell lysate. Use 20ug per lane.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~3mg/ml
Form	Supplied as a liquid in PBS, 30% glycerol.
Purity	Purified by Protein A-Sepharose chromatography.
Immunogen	Synthetic peptide (ELVQKAKLAEQA) corresponding to the beta and zeta isoforms of 14–3–3.
Specificity	Recognizes beta and zeta isoforms in Drosophila and mammalian systems.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.