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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, beta, zeta

Anti -14-3-3, beta, zeta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr.
Catalog #0014-03
ApplicationsSuitable for use in Western Blot. No recommended for use in Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1ug/ml detected 14-3-3 protein in a RIPA lysate prepared from human A431 epidermoid (A0001-50), mouse 3T3 fibroblast, rat L6 skeletal fibroblast, chinese hamster ovary cells and rabbit brain cytosol.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Included Positive ControlA0001-50: Non-stimulated A431 cell lysate. Use 20ug per lane.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~3mg/ml
FormSupplied as a liquid in PBS, 30% glycerol.
PurityPurified by Protein A-Sepharose chromatography.
ImmunogenSynthetic peptide (ELVQKAKLAEQA) corresponding to the beta and zeta isoforms of 14𣛩.
SpecificityRecognizes beta and zeta isoforms in Drosophila and mammalian systems.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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