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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3E, NT (YWHAE, 14-3-3 Protein epsilon)

Anti -14-3-3E, NT (YWHAE, 14-3-3 Protein epsilon)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
14-3-3 epsilon is a member of the 14-3-3 family which consists of 30kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.
Catalog #0014-04H
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceSheep
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon.
SpecificityRecognizes sheep YWHAE. Species sequence homology: Bovine, chicken, human, mouse, rabbit, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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