Anti -14-3-3, gamma (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine
3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP |
|
| 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer’s disease and Down syndrome level of 14-3-3 gamma in brain increases significantly. | | | Catalog # | 0014-07B | | Applications | Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. | | Recommended Dilutions | Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein G. | | Western Blot: 1-2ug/ml for 2 hrs at RT. | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3F8 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See 0014-07BX. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | 14-3-3 gamma recombinant protein of human origin. MW | | Specificity | Recognizes human 14-3-3 gamma. Does not crossreact with other isoforms of 14-3-3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG |
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