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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, gamma, ID (Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein gamma, N-terminally Processed, YWHAG)

Anti -14-3-3, gamma, ID (Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein
gamma, N-terminally Processed, YWHAG)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The initially described alpha and delta isoforms are confirmed to be phosphorylated forms of beta and zeta respectively. Through their amino-terminal alpha helical region, 14-3-3 proteins forms homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes which modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in develop- ment and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform differ- ent functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Catalog #0014-08C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone NoJ3H10
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified
ImmunogenRecombinant corresponding to human 14-3-3 gamma aa1-247, expressed in E. coli.
SpecificityRecognizes human YWHAG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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