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You are here:Home » Antibodies » Antibodies-14-3-3 » Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)

Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner.
14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.
Catalog #0014-19
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 0014-19. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlA0001-55: EGF-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No1.T.28
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenGST fusion protein corresponding to full-length human 14-3-3 sigma
SpecificityRecognizes human 14-3-3 sigma, Mr 30kD. Does not crossreact with other 14-3-3 isoforms. Species Crossreactivity: rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSFN, Stratifin, HGNC:10773

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