Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| 14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells. | | | Catalog # | 0014-19A | | Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilutions | Immunoprecipitation: 2ug/mg protein lysate. Use Protein G. | | Western Blot: 1ug/ml for 2 hours at RT. | | Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. | | Optimal dilutions to be determined by the researcher. | | Positive Control | HT29 cells and skin | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 1.N.6 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide. See 0014-07A. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant human 14-3-3 sigma protein. | | Cellular Localization | Cytoplasmic. | | Specificity | Recognizes human 14-3-3 sigma protein (26-30kD). Does not crossreact with other isoforms of 14-3-3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SFN, Stratifin, HGNC:10773 |
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