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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)

Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer’s disease and Down syndrome level of 14-3-3 gamma in brain increases significantly.
Catalog #0014-19B
Gene ID(s) 2810 (human)
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA (Peptide): 1:64,000.
Western Blot: 0.1-0.3ug/ml. ~ 28kD band observed in Human Skin lysates (calculated MW of 27.8kD according to NP_006133.1).
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: C-DLHTLSEDSYKDST, representing the internal region of the human protein according to NP_006133.1.
SpecificityRecognizes human 14-3-3 sigma . Species sequence homology: mouse, rat, canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSFN, Stratifin, HGNC:10773


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