Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer’s disease and Down syndrome level of 14-3-3 gamma in brain increases significantly. | | | Catalog # | 0014-19B | | Gene ID(s) | 2810 (human) | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA (Peptide): 1:64,000. | | Western Blot: 0.1-0.3ug/ml. ~ 28kD band observed in Human Skin lysates (calculated MW of 27.8kD according to NP_006133.1). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | | | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide: C-DLHTLSEDSYKDST, representing the internal region of the human protein according to NP_006133.1. | | Specificity | Recognizes human 14-3-3 sigma . Species sequence homology: mouse, rat, canine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SFN, Stratifin, HGNC:10773 |
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