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You are here:Home » Antibodies » Antibodies-14-3-3 » Anti -14-3-3, sigma (14-3-3 Protein sigma, Stratifin, SFN, Epithelial Cell Marker Protein 1, Er, HGNC:10773, HME1, Mme1, OTTHUMP00000004242, YWHAS)

Anti -14-3-3, sigma (14-3-3 Protein sigma, Stratifin, SFN, Epithelial Cell Marker Protein
1, Er, HGNC:10773, HME1, Mme1, OTTHUMP00000004242, YWHAS)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
Catalog #0014-19C
14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner.
14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:64000
Western Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in Tris-saline, 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide of 14-3-3 sigma.
SpecificityRecognizes human 14-3-3 sigma at ~28kD in human skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Species Crossreactivity: Mouse, rat and dog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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