| The 14-3-3 proteins are a family of homologous, evolutionarily conserved, acidic proteins expressed in all eukaryotes, with molecular masses ranging from 28-33kD. To date, seven different 14-3-3 isoforms have been identified in mammals, each encoded by a distinct gene. The expression of 14-3-3 isoforms is tissue specific and differentially regulated. The 14-3-3 proteins assemble into stable homo- and heterodimers, which modulate signaling cascades by binding to phosphorylated serine or threonine residues of target proteins. In addition to a phosphorylated serine or phohsphorylated threonine residue, 14-3-3 protein binding also requires an arginine located at position –3 or –4. The 14-3-3 proteins affect signaling in several manners, including: interfering with protein/protein interactions (example: 14-3-3’s interference |
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| with the BAD/Bcl-2 interaction, inhibiting apoptosis); modulating target nuclear transport (examples: 14-3-3’s modulation of nuclear transport of Cdc25, telomerase and forkhead transcription factors); modulating target catalytic activity (example: 14-3-3’s binding to tryptophan hydrolase, enhancing its catalytic activity); and protecting targets from proteolyis (examples: 14-3-3’s protection of Raf and BAD). The 14-3-3 proteins also modulate signaling cascades by serving as scaffolding/adapter proteins. The measurement of 14-3-3 isoforms in cerebrospinal fluid has utility in diagnosing prion diseases such as Creutzfeldt-Jakob disease. |
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| Catalog # | 0014-21 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation of both the native and denatured forms of the protein. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Positive Controls | PC12 cells, 3T3 cells, MCBK cells, Jurkat cells, or normal human fibroblasts. |
| Hybridoma | Balb/c splenocytes |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5J20 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 20mM sodium phosphate, 150mM sodium chloride, pH 7.5, 0.08% sodium azide, 50% glycerol. |
| Purity | Purified |
| Immunogen | Recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3 theta and 14-3-3 tau). |
| Specificity | Recognizes human 14-3-3 theta/tau. Does not crossreact with 14-3-3 zeta. 14-3-3 theta/tau is expressed by brain tissue and cells of the male germ line. Species Crossreactivity: mouse, rat and bovine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
