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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3 theta (14-3-3 tau)

Anti -14-3-3 theta (14-3-3 tau)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
The 14-3-3 proteins are a family of homologous, evolutionarily conserved, acidic proteins expressed in all eukaryotes, with molecular masses ranging from 28-33kD. To date, seven different 14-3-3 isoforms have been identified in mammals, each encoded by a distinct gene. The expression of 14-3-3 isoforms is tissue specific and differentially regulated. The 14-3-3 proteins assemble into stable homo- and heterodimers, which modulate signaling cascades by binding to phosphorylated serine or threonine residues of target proteins. In addition to a phosphorylated serine or phohsphorylated threonine residue, 14-3-3 protein binding also requires an arginine located at position 3 or 4. The 14-3-3 proteins affect signaling in several manners, including: interfering with protein/protein interactions (example: 14-3-3s interference
with the BAD/Bcl-2 interaction, inhibiting apoptosis); modulating target nuclear transport (examples: 14-3-3s modulation of nuclear transport of Cdc25, telomerase and forkhead transcription factors); modulating target catalytic activity (example: 14-3-3s binding to tryptophan hydrolase, enhancing its catalytic activity); and protecting targets from proteolyis (examples: 14-3-3s protection of Raf and BAD). The 14-3-3 proteins also modulate signaling cascades by serving as scaffolding/adapter proteins. The measurement of 14-3-3 isoforms in cerebrospinal fluid has utility in diagnosing prion diseases such as Creutzfeldt-Jakob disease.
Catalog #0014-21
ApplicationsSuitable for use in Western Blot and Immunoprecipitation of both the native and denatured forms of the protein.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive ControlsPC12 cells, 3T3 cells, MCBK cells, Jurkat cells, or normal human fibroblasts.
HybridomaBalb/c splenocytes
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5J20
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 20mM sodium phosphate, 150mM sodium chloride, pH 7.5, 0.08% sodium azide, 50% glycerol.
PurityPurified
ImmunogenRecombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3 theta and 14-3-3 tau).
SpecificityRecognizes human 14-3-3 theta/tau. Does not crossreact with 14-3-3 zeta. 14-3-3 theta/tau is expressed by brain tissue and cells of the male germ line. Species Crossreactivity: mouse, rat and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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