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You are here:Home » Antibodies » Antibodies-14-3-3 » Anti -14-3-3 zeta (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)

Anti -14-3-3 zeta (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1,


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
14-3-3 zeta is a 245aa containing protein belonging to the 14-3-3 family mediating signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.
Catalog #0014-22H
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 3-5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlBrain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa50-100 of human 14-3-3 zeta.
SpecificityRecognizes human 14-3-3 zeta. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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