Anti -14-3-3, zeta, gamma, eta (Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine
3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| 14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and hetero-dimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. At least seven isoforms of 14-3-3 have been identi ed with the zeta isoform being the most broadly expressed in animals. | | | Catalog # | 0014-25 | | Applications | Suitable for use in ELISA and Western Blot. Not recommended for use in Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:5000-1:10,000 dilution detected 14-3-3 protein in a preparation of bovine brain cytosol. | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb | | I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb | | I1904-46Q: IgG, H&L (HRP) Pab Gt xRb | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | Not determined | | Form | Supplied as a liquid. No preservatives added. | | Purity | Neat serum | | Immunogen | HPLC-puri ed synthetic peptide (KMKGDYYRYLAE) corresponding to the zeta, gamma and eta isoforms of 14-3-3. | | Specificity | Recognizes zeta, gamma and eta isoforms at 27kD in various mammalian systems. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG |
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