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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, zeta, gamma, eta (Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG)

Anti -14-3-3, zeta, gamma, eta (Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine
3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and hetero-dimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. At least seven isoforms of 14-3-3 have been identi ed with the zeta isoform being the most broadly expressed in animals.
Catalog #0014-25
ApplicationsSuitable for use in ELISA and Western Blot. Not recommended for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-1:10,000 dilution detected 14-3-3 protein in a preparation of bovine brain cytosol.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid. No preservatives added.
PurityNeat serum
ImmunogenHPLC-puri ed synthetic peptide (KMKGDYYRYLAE) corresponding to the zeta, gamma and eta isoforms of 14-3-3.
SpecificityRecognizes zeta, gamma and eta isoforms at 27kD in various mammalian systems.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesProtein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG


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