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You are here:Home » Kits and Assays » Kits-ELISA, BioAssay™ » Acrosin (ACR) BioAssay™ ELISA Kit (Human)

Acrosin (ACR) BioAssay™ ELISA Kit (Human)


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Acrosin (ACR) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACR in human sperm.
Catalog #023089
BackgroundACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Detection Range0.156-10ng/ml
Kit Components023089A: Microtiter Plate, 1x96 wells
023089B: Standard (lyophilized), 2x1vial
023089C: Detection Reagent A (100X), 1x120ul
023089D: Detection Reagent B (100X), 1x120ul
023089E: TMB Substrate, 1x9ml
023089F: Wash Buffer (30X), 1x20ml
023089G: Standard Diluent, 1x20ml
023089H: Assay Diluent A (2X), 1x6ml
023089J: Assay Diluent B (2X), 1x6ml
023089K: Stop Solution, 1x6ml
Storage and StabilityStore 023089A, 023089B, 023089C, 023089D at -20°C upon receipt. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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