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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Actin alpha 1, Skeletal Muscle (ACTa1) BioAssay™ ELISA Kit (Multi species)

Actin alpha 1, Skeletal Muscle (ACTa1) BioAssay™ ELISA Kit (Multi species)

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Specifications

Actin Alpha 1, Skeletal Muscle (ACTa1) BioAssay™ ELISA Kit (Multi) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTa1 in human, mouse, rat, rabbit, bovine and porcine tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The sequence of ACTa1 recognized by this kit has 100% homology with mouse, rat, rabbit, bovine and porcine.
Catalog #023090
Detection Range1.56-100ng/ml
Sensitivity0.63ng/ml
Intra-Assay CV<10%
Inter-Assay CV<12%
Kit Components*023090A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*023090B: Standard, 2x1 vial
023090C: Standard Diluent, 1x20ml
*023090D: Detection Reagent A, 1x120ul
*023090E: Detection Reagent B, 1x120ul
023090F: Assay Diluent A, 1x12ml
023090G: Assay Diluent B, 1x12ml
023090H: TMB Substrate, 1x9ml
023090K: Stop Solution, 1x6ml
023090L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (023090K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023090A, *023090B, *023090D and *023090E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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