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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Actin alpha 2, Smooth Muscle (ACTa2) BioAssay™ ELISA Kit (Human)

Actin alpha 2, Smooth Muscle (ACTa2) BioAssay™ ELISA Kit (Human)

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Specifications

Actin Alpha 2, Smooth Muscle (ACTa2) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTa2 in human serum, plasma and other biological fluids.
Catalog #023092
Detection Range7.813-500ng/ml
Sensitivity~1.7ng/ml
Intra-AssayCV<10%
Inter-AssayCV<12%
Kit Components*023092A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
*023092B: Standard, 2x1vial 
023092C: Standard Diluent, 1x20ml
*023092D: Detection Reagent A, 1x120ul 
*023092: Detection Reagent B, 1x120ul 
023092F: Assay Diluent A, 2x, 1x6ml
023092G: Assay Diluent B, 2x, 1x6ml
023092H: TMB Substrate, 1x9ml
023092J: Positive Control, 28.69ng/ml, 1x1vial
023092K: Stop Solution, 1x6ml
023092L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (026229K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Notes on 023092J - Positive ControlIntended Use:
The control vials are from human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
CharacteristicsThe control vials contain human ACTa2 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Values28.69ng/ml
The concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.
UsageDisslove with 150ul standard dilutent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Storage and StabilityStore *023092A, *023092B, *023092D and *023092E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.


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