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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Actin Beta (ACTb) BioAssay™ ELISA Kit

Actin Beta (ACTb) BioAssay™ ELISA Kit

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Specifications

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTb in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 100% sequence homologyamong different species, the kit can be used to detect human, mouse and rat samples.
Catalog #023094
Detection Range1.56-100ng/ml
Sensitivity0.63ng/ml
Intra-Assay CV<10%
Inter-Assay CV<12%
Kit Components*023094A: Microtiter Plate, 1x96wells, Pre-coated, ready to use
*023094B: Standard, 2x1vial
023094C: Standard Diluent, 1x20ml
*023094D: Detection Reagent A, 1x120ul
*023094E: Detection Reagent B, 1x120ul
023094F: Assay Diluent A, 2x, 1x6ml
023094G: Assay Diluent B, 2x, 1x6ml
023094H: TMB Substrate, 1x9ml
023094K: Stop Solution, 1x6ml
023094L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (023094K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023094A, *023094B, *023094D and *023094E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul 023094H: TMB Substrate. Incubate 15-25 minutes at 37°C.
7. Add 50ul 023094K: Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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