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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay™ ELISA Kit (Human)

Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay™ ELISA Kit
(Human)

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Specifications

Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay™ ELISA Kit (Human) is a competitive inhibition enzyme immunoassay for in vitro quantitative measurement of ADCYAP1 in human serum, plasma, tissue homogenates and other biological fluids.
Catalog #023137
Detection Range6.17-500pg/ml
Sensitivity2.62pg/ml
Intra-Assay CV<10%
Inter-Assay CV<12%
Kit Components*023137A: Microtiter Strips, 1x96 wells
*023137B: Standard, 2x1 vial
023137C: Standard Diluent, 1x20ml
*023137D: Detection Reagent A, 1x120ul
*023137E: Detection Reagent B, 1x120ul
023137F: Assay Diluent A, 1x12ml
023137G: Assay Diluent B, 1x12ml
023137H: TMB Substrate, 1x9ml
023137K: Stop Solution, 1x6ml
023137L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (023137K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023137A, *023137B, *023137D and *023137E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
7. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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