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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Advanced Glycation End Product (AGE) BioAssay™ ELISA Kit

Advanced Glycation End Product (AGE) BioAssay™ ELISA Kit

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Specifications

The Advanced Glycation End Product (AGE) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of AGE in serum, plasma and other biological fluids.
Catalog #023185
Detection Range98.8-8,000ng/ml
Sensitivity33.6ng/ml
PrecisionIntra-Assay CV: <10%
Inter-Assay CV: <12%
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for AGE has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled AGE and unlabeled AGE (standards or samples) with the pre-coated antibody specific for AGE. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of AGE in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of AGE in the sample.
Kit Components*023185A: Microtiter Plate, 96 wells, Pre-coated, ready to use
*023185B: Standard, 2x1vial
023185C: Standard Diluent, 1x20ml
*023185D: Detection Reagent A, 1x120ul
*023185E: Detection Reagent B, 1x120ul
023185F: Assay Diluent A, 1x12ml
023185G: Assay Diluent B, 1x12ml
023185H: TMB Substrate, 1x9ml
023185K: Stop Solution, 1x6ml
023185L: Wash Buffer, 30X, 1x20ml
Storage and StabilityStore *023185A, *023185B, *023185D and *023185E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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