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You are here:Home » Kits and Assays » Kits-ELISA (Oxidative Stress), BioAssay™ » Advanced Glycosylation End Product Specific Receptor (AGER) BioAssay™ ELISA Kit (Mouse)

Advanced Glycosylation End Product Specific Receptor (AGER) BioAssay™ ELISA Kit (Mouse)


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The Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AGER in mouse serum, plasma, tissue homogenates, cell lysates, celll culture supernatants and other biological fluids.
Catalog #023187
Detection Range0.156-10ng/ml
PrecisionIntra-Assay: CV<10%
Inter-Assay: CV<12%
Test PrincipleThe microtiter plate provided in this kit has been pre-coated with an antibody specific to AGER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGER in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components*023187A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use
*023187B: Standard, 2x1vial
023187C: Standard Diluent, 1x20ml
*023187D: Detection Reagent A, 1x120ul
*023187E: Detection Reagent B, 1x120ul
023187F: Assay Diluent A, 1x12ml
023187G: Assay Diluent B, 1x12ml
023187H: TMB Substrate, 1x9ml
023187K: Stop Solution, 1x6ml
023187L: Wash Buffer, 30x, 1x20ml
Storage and StabilityStore *023187A, *023187B, *023187D and *023187E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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