Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Afamin (AFM) BioAssay™ ELISA Kit (Human)

Afamin (AFM) BioAssay™ ELISA Kit (Human)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Afamin (AFM) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of AFM in human serum, plasma and other biological fluids.
Catalog #023191
Detection Range93.75-6000pg/ml
Sensitivity42.51pg/ml
Intra-Assay CV< 10%
Inter-Assay CV< 12%
Test PrincipleThe microtiter plate provided in this kit has been pre-coated with an antibody specific to AFM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AFM in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components*023191A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*023191B: Standard, 2x1vial
023191C: Standard Diluent, 1x20ml
*023191D: Detection Reagent A, 1x120ul
*023191E: Detection Reagent B, 1x120ul
023191F: Assay Diluent A, 1x12ml
023191G: Assay Diluent B, 1x12ml
023191H: TMB Substrate, 1x9ml
023191K: Stop Solution, 1x6ml
023191L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (023191K) provided for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023191A, *023191B, *023191D and *023191E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links