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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Agouti Related Protein (AGRP) BioAssay™ ELISA Kit (Mouse)

Agouti Related Protein (AGRP) BioAssay™ ELISA Kit (Mouse)

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Specifications

The Mouse Agouti Related Protein (AGRP) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AGRP in mouse serum, plasma and other biological fluids.
Catalog #023199
Detection Range4.69-300pg/ml
Sensitivity1.83pg/ml
Intra-AssayCV<10%
Inter-AssayCV<12%
Kit Components*023199A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use
*023199B: Standard, 2x1vial
023199C: Standard Diluent, 1x20ml
*023199D: Detection Reagent A (green), 1x120ul
*023199E: Detection Reagent B (red), 1x120ul
023199F: Assay Diluent A, 1x12ml
023199G: Assay Diluent B, 1x12ml
023199H: TMB Substrate, 1x9ml
023199K: Stop Solution, 1x6ml
023199L: Wash Buffer, 30X, 1x20ml
Storage and StabilityStore *023199A, *023199B, *023199D and *023199E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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