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You are here:Home » Kits and Assays » Kits-ELISA, BioAssay™ » Agouti Related Protein (AGRP) BioAssay™ ELISA Kit (Mouse)

Agouti Related Protein (AGRP) BioAssay™ ELISA Kit (Mouse)

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Specifications

The Agouti Related Protein (AGRP) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AGRP in mouse serum, plasma and other biological fluids.
Catalog #023199
Detection Range4.69-300pg/ml
Sensitivity1.92pg/ml
Intra-AssayCV<10%
Inter-AssayCV<12%
Kit Components*023199A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated; ready to use
*023199B: Standard, 2x1vial
023199C: Standard Diluent, 1x10ml or 1x20ml
*023199D: Detection Reagent A (green), 1x60ul or 1x120ul
*023199E: Detection Reagent B (red), 1x60ul or 1x120u
023199F: Assay Diluent A, 1x6ml or 1x12ml
023199G: Assay Diluent B, 1x6ml or 1x12ml
023199H: TMB Substrate, 1x4.5ml or 1x9ml
*023199J: Positive Control, 19.8pg/ml, 1x1vial
023199K: Stop Solution, 1x3ml or 1x6ml
023199L: Wash Buffer, 30X, 1x10ml or 1x20ml
PrecautionThe Stop Solution (023199K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023199A, *023199B, *023199D, *023199E and *023199J at -20°C. Store all the other components at 4°C. After reconstitution of *023199J, store at 4°C and use within 5 days. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 03199J Positive Control:
Intended UseThe control vial is from a mouse serum sample known to contain AGRP. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
CharacteristicsThe control vial contains mouse AGRP in mouse serum (lyophilized powder). Note: The mouse source material used in this product is from healthy mouse of SPF standards (specific pathogen-free animals).
Expected Values19.8pg/ml
UsageDissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
SourceMouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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