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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Agrin (AGRN) BioAssay™ ELISA Kit (Human)

Agrin (AGRN) BioAssay™ ELISA Kit (Human)

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Specifications

Agrin (AGRN) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of AGRN in human serum, plasma, tissue homogenates and other biological fluids.
Catalog #023200
Detection Range31.2-2000pg/ml
Sensitivity13.6pg/ml
Intra-Assay CV<10%
Inter-Assay CV<12%
Kit Components*023200A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*023200B: Standard, 2x1 vial
023200C: Standard Diluent, 1x20ml
*023200D: Detection Reagent A (green), 1x120ul
*023200E: Detection Reagent B (red), 1x120ul
023200F: Assay Diluent A, 1x12ml
023200G: Assay Diluent B, 1x12ml
023200H: TMB Substrate, 1x9ml
023200K: Stop Solution, 1x6ml
023200L: Wash Buffer, 30X, 1x20ml
PrecautionThe Stop Solution (023200K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and StabilityStore *023200A, *023200B, *023200D and *023200E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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