15.6-1000pg/ml

6.4pg/ml

<10%

<12%

*026500A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use *026500B: Standard, 2x1 vial 026500C: Standard Diluent, 1x10ml or 1x20ml *026500D: Detection Reagent A (green), 1x60ul or 1x120ul *026500E: Detection Reagent B (red), 1x60ul or 1x120ul 026500F: Assay Diluent A, 1x6ml or 1x12ml 026500G: Assay Diluent B, 1x6ml or 1x12ml 026500H: TMB Substrate, 1x4.5ml or 1x9ml *026500J: Positive Control, 425.4pg/ml, 1x1vial 026500K: Stop Solution, 1x3ml or 1x6ml 026500L: Wash Buffer, 30X, 1x10ml or 1x20ml

The Stop Solution (026500K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *026500A, *026500B, *026500D, *026500E and *026500J at -20°C. After reconstitution of *026500J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

The control vials are from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

The control vial contains rat LIF in rat serum (lyophilized powder). Note: The rat based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).

425.4pg/ml

Dissolve with 150ul standard diluent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.

Rat

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.