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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Lipopolysaccharides (LPS) BioAssay™ ELISA Kit (Lipoglycan, Endotoxin)

Lipopolysaccharides (LPS) BioAssay™ ELISA Kit (Lipoglycan, Endotoxin)

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Specifications

Lipopolysaccharide (LPS) is one of the main components of Gram-negative bacteria, which is also known as endotoxin. LPS can cause fever, shock, and even lead to death. With the growing popularity of genetic engineering techniques in biological products and reagents, LPS becomes an important indicator to monitor residual bacteria as well as pyrogenicity in bio-products. This LPS BioAssay™ ELISA Kit has been designed using proprietary hybridoma technology and is an simpler alternative to the traditional LAL (Limulus Amebocyte Lysate) agglutination assay. Following is a comparison between our LPS ELISA Kit and LAL assay:
Catalog #026552
LAL Agglutination LPS ELISA Principle LAL reacts with endotoxin Specific antibodyantigen interaction
Quantitation Semi, visual Specific, microplate reader
Throughput 1 88
Vector Test tube 96 well plate
Dilution Multiple Single
Calculation Complex Simple
Sample Volume >100ul 50ul
The Lipopolysaccharide (LPS) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay for the in vitro quantitative measurement of LPS in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Detection Range12.35-1000ng/ml
Sensitivity 4.68ng/ml
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for LPS has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled LPS and unlabeled LPS (standards or samples) with the pre-coated antibody specific for LPS. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of LPS in the sample. After addition of the substrate solution, the intensity of the color developed is inversely proportional to the concentration of LPS in the sample.
Kit Components*026552A: Microtiter Strips, 96-well strip plate. Pre-coated, ready to use
*026552B: Standard, 2x1vial
026552C: Standard Diluent, 1x20ml
*026552D: Detection Reagent A, 1x120ul
*026552E: Detection Reagent B, 1x120ul
026552F: Assay Diluent A, 1x12ml
026552G: Assay Diluent B, 1x12ml
026552H: TMB Substrate, 1x9ml
026552K: Stop Solution, 1x6ml
026552L: Wash Buffer, 30X, 1x20ml
Storage and StabilityStore *026552A, *026552B, *026552D and *026552E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
7. Add 50ul Stop Solution. Read at 450nm immediately.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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