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You are here:Home » Antibodies » Antibodies-Nucleotides » Anti -5-Methylcytosine (5mC, 5-Methyl-Cytosine, 5 MethylCytidine, 5 MeCyd, 5 Me Cytidine)

Anti -5-Methylcytosine (5mC, 5-Methyl-Cytosine, 5 MethylCytidine, 5 MeCyd, 5 Me Cytidine)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified
5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT's). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
Catalog #029406
ApplicationsSuitable for use in Dot Blot. Other applications not tested.
Recommended DilutionDot Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlMethylated and unmethylated BRCA1
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No13A149
FormSupplied as a liquid in PBS. No preservatives added.
PurityPurified by Protein A affinity chromatography.
Immunogen5-methylcytosine conjugated to Ovalbumin
SpecificityRecognizes human 5-Methylcytosine. Species sequence homology: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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