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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -ACTN4, NT (Alpha-actinin-4, F-actin Cross-linking Protein, Non-muscle alpha-actinin 4)

Anti -ACTN4, NT (Alpha-actinin-4, F-actin Cross-linking Protein, Non-muscle alpha-actinin
4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins. Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm. It plays a role in cell motility, invasion, and metastasis of cancer cells. Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion.
Catalog #029408
ApplicationsSuitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (paraffin): 1:100-1:250
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlHuman fetal skeletal muscle tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A151
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to human ACTN4 at N-terminal.
SpecificityRecognizes human ACTN4. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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