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You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12, APG12L)

Anti -ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like,
APG12, APG12L)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy. It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles. The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required.
Catalog #029420
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Positive ControlFetal kidney tissue lysate, HepG2 and Raji cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A158
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to human ATG12 at C-terminal.
SpecificityRecognizes human ATG12. Species sequence homology: Mouse and rat (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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