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You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG4A (Cysteine Protease ATG4A, AUT-like 2 Cysteine Endopeptidase, Autophagin-2, Autophagy-related Cysteine Endopeptidase 2, Autophagy-related Protein 4 Homolog A, hAPG4A, APG4A, AUTL2)

Anti -ATG4A (Cysteine Protease ATG4A, AUT-like 2 Cysteine Endopeptidase, Autophagin-2,
Autophagy-related Cysteine Endopeptidase 2, Autophagy-related Protein 4 Homolog A, hAPG4A,
APG4A, AUTL2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of target proteins, such as MAP1LC3, GABARAPL2 and GABARAP. ATG4A belongs to the peptidase C54 family, and is widely with high expression level in skeletal muscle and brain.
Catalog #029421
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-10,000
Immunoprecipitation: 1:10-1:100
Immunohistochemistry (paraffin): 1:100-1:250
Optimal dilutions to be determined by the researcher.
Positive ControlEvaluated by Western Blot in fetal liver, HepG2, Jurkat, and fetal brain lysates.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A159
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to human ATG4A.
SpecificityRecognizes human ATG4A. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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