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You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG4D, NT (Cysteine Protease ATG4D, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, APG4D, AUTL4)

Anti -ATG4D, NT (Cysteine Protease ATG4D, AUT-like 4 Cysteine Endopeptidase, Autophagin-4,
Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, APG4D,
AUTL4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Atg4D is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.
Catalog #029422
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic linear peptide corresponding to the N-terminus of human ATG4D (KLH).
SpecificityRecognizes human ATG4D. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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