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You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP)

Anti -ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Atg5 (Autophagy protein 5) is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Atg5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Catalog #029423
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-10,000
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (paraffin): 1:100-1:250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.
Positive ControlRaji, HeLa, HT-1080, fetal kidney, C6, Raw264.7, PC-12, and NIH/3T3 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A160
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to human ATG5.
SpecificityRecognizes human ATG5. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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