Anti -Calreticulin (CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60,
HACBP, grp60, CALR, CRTC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC FC |
|
| Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters. Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER. Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression. | | | Catalog # | 029433 | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution Western Blot | 1:1000-1:10,000 | | Immunoprecipitation: 1:10 | | Flow Cytometry: 1:10 | | Immunohistochemistry: 1:250-1:500 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Positive Control | SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 13A162 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to human Calreticulin. | | Specificity | Recognizes human Calreticulin. Species Crossreactivity: Mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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