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Abs to Enzymes, Methyltransferase
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Anti -Ezh2, Phosphorylated (Thr487) (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6)
Anti -Ezh2, Phosphorylated (Thr487) (Enhancer of Zeste Homolog 2, Histone-lysine
N-methyltransferase EZH2, ENX1, ENX-1, KMT6)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH |
|
| Enhancer of Zeste homolog 2 is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes. Ezh2 interacts specifically with Vav both in vitro and in vivo. Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development. | | | Catalog # | 029469 | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. | | Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:10,000 | | Immunoprecipitation: 1:10 | | Immunohistochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 13A182 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in buffer, 0.01% sodium azide, 0.05% BSA, 50% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to human Ezh2 phosphorylated at Thr487. | | Specificity | Recognizes human Ezh2 phosphorylated at Thr487. Species Crossreactivity: Mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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