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You are here:Home » Antibodies » Antibodies-Histones » Anti -H2AFX, phosphorylated (Ser139) (Histone H2A.x, H2a/x, H2AX)

Anti -H2AFX, phosphorylated (Ser139) (Histone H2A.x, H2a/x, H2AX)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
Catalog #029491
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-10,000
Immunocytochemistry: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlEtoposide untreated and treated Jurkat cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No13A191
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
ImmunogenSynthetic peptide corresponding to human Histone H2A.X phosphorylated at Ser139.
SpecificityRecognizes human Histone H2A.X phosphorylated at Ser139. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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