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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, Monomethyl, Dimethyl, Trimethyl (Lys4) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, H

Anti -Histone H3, Monomethyl, Dimethyl, Trimethyl (Lys4) (Histone H3.1, H3/a, H3/b, H3/c,
H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC,
HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, H

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.
Catalog #029498
ApplicationsSuitable for use in Western Blot and Cell Function Assay. Other applications not tested.
Recommended DilutionWestern Blot: 1:8000
Optimal dilutions to be determined by the researcher.
Positive ControlRecombinant Histone H3 and HeLa acid extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A193
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 40% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic linear peptide corresponding to human Histone H3 mono/di/tri-methylated at Lys4 (KLH).
SpecificityRecognizes human Histone H3 mono/di/tri-methylated at Lys4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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