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You are here:Home » Antibodies » Abs to Histones » Anti -HIstone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B)

Anti -HIstone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
Catalog #029502
ApplicationsSuitable for use in Western Blot, Chromatin Immunoprecipitation, Dot Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa acid extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic linear peptide corresponding to human Histone H3.3 (KLH).
SpecificityRecognizes human Histone H3.3. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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