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You are here:Home » Antibodies » Antibodies-Nuclear Proteins » Anti -HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M, hnRNP M, HNRPM, NAGR1)

Anti -HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M, hnRNP M, HNRPM, NAGR1)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
Catalog #029506
ApplicationsSuitable for use in Western Blot and RNA Binding Protein Immunoprecipitation assay. Other applications not tested.
Recommended DilutionRIP: ~5ug of antibody per RIP
Optimal dilutions to be determined by the researcher.
Positive ControlIncludes negative control normal mouse IgG antibody and control primers specific for the cDNA of human hnRNP M.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No13A197
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenT7 gene 10 fusion protein containing full length human RNP M4.
SpecificityRecognizes human HNRNPM. Species sequence homology: Bovine, mouse, porcine, rat, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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