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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Catalog #	029552
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Positive Control	U251 cells
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1k
Clone No	13A210
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with R-Phycoerythrin (PE).
Purity	Purified by Protein A affinity chromatography.
Immunogen	Fusion protein.
Specificity	Recognizes human Nestin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.