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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (NES, Nbla00170) (PE)

Anti -Nestin (NES, Nbla00170) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Catalog #029552
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive ControlU251 cells
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No13A210
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein A affinity chromatography.
ImmunogenFusion protein.
SpecificityRecognizes human Nestin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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