Anti -SIRP alpha (Signal Regulatory Protein alpha, SIRPalpha, SIRPA, SIRP,
Signal-regulatory Protein alpha-1, Sirp-alpha-1, Signal-regulatory Protein alpha-2,
Sirp-alpha-2, Signal-regulatory Protein alpha-3, Sirp-alpha-3, Brain Ig-like Molecule with
Tyrosine-ba
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
B IH IC |
|
| SHPS-1 (also known as P84, BIT, and SIRP) is a neuronal membrane glycoprotein that promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. Because of its heterophilic adhesive properties and its localization at synaptogenesis sites, SHPS-1 is suggested play a role in the regulation of synapse formation or maintenance. As a member of a phosphatase binding protein family, SHPS-1 shares identical homology with murine SHPS-1 cDNA. It is involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. It is reported that integrin-associated protein (IAP/CD47) is a predominant binding partner for P84. Upon phosphorylation of its cytoplasmic region, SHPS-1 binds to the protein-tyrosine phosphatase SHPS-2. Formation of the SHPS-1-SHP-2 complex is also implicated in the regulation of cellular migration. SHPS-1 and its ligand CD47 constitute an intercellular recognition system that contributes to the inhibition of cell migration via cell-cell contact. The ectodomain of SHPS-1 is shown to be excised from cells in a reaction that is likely mediated by a metalloproteinase. It is demonstrated that forced expression of a SHPS-1 mutant resistant to ectodomain shedding impaires cell migration, cell spreading, actin cytoskeleton reorganization, and paxillin- and FAK- tyrosine phosphorylation. | | | Catalog # | 029609 | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Mouse brain tissue lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IGg1k | | Clone No | 13A218 | | Host | Rat | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic linear peptide corresponding to mouse SHPS-1. | | Specificity | Recognizes mouse SHPS-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
|
Questions or comments about this product? Inquire here. |
|
|
Download Specifications for Anti -SIRP alpha (Signal Regulatory Protein alpha, SIRPalpha, SIRPA, SIRP, Signal-regulatory Protein alpha-1, Sirp-alpha-1, Signal-regulatory Protein alpha-2, Sirp-alpha-2, Signal-regulatory Protein alpha-3, Sirp-alpha-3, Brain Ig-like Molecule with Tyrosine-ba |
External Links
|
