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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -SIRP alpha (Signal Regulatory Protein alpha, SIRPalpha, SIRPA, SIRP, Signal-regulatory Protein alpha-1, Sirp-alpha-1, Signal-regulatory Protein alpha-2, Sirp-alpha-2, Signal-regulatory Protein alpha-3, Sirp-alpha-3, Brain Ig-like Molecule with Tyrosine-ba

Anti -SIRP alpha (Signal Regulatory Protein alpha, SIRPalpha, SIRPA, SIRP,
Signal-regulatory Protein alpha-1, Sirp-alpha-1, Signal-regulatory Protein alpha-2,
Sirp-alpha-2, Signal-regulatory Protein alpha-3, Sirp-alpha-3, Brain Ig-like Molecule with
Tyrosine-ba

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH IC
SHPS-1 (also known as P84, BIT, and SIRP) is a neuronal membrane glycoprotein that promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. Because of its heterophilic adhesive properties and its localization at synaptogenesis sites, SHPS-1 is suggested play a role in the regulation of synapse formation or maintenance. As a member of a phosphatase binding protein family, SHPS-1 shares identical homology with murine SHPS-1 cDNA. It is involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. It is reported that integrin-associated protein (IAP/CD47) is a predominant binding partner for P84. Upon phosphorylation of its cytoplasmic region, SHPS-1 binds to the protein-tyrosine phosphatase SHPS-2. Formation of the SHPS-1-SHP-2 complex is also implicated in the regulation of cellular migration. SHPS-1 and its ligand CD47 constitute an intercellular recognition system that contributes to the inhibition of cell migration via cell-cell contact. The ectodomain of SHPS-1 is shown to be excised from cells in a reaction that is likely mediated by a metalloproteinase. It is demonstrated that forced expression of a SHPS-1 mutant resistant to ectodomain shedding impaires cell migration, cell spreading, actin cytoskeleton reorganization, and paxillin- and FAK- tyrosine phosphorylation.
Catalog #029609
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlMouse brain tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIGg1k
Clone No13A218
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic linear peptide corresponding to mouse SHPS-1.
SpecificityRecognizes mouse SHPS-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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