MYL5, Recombinant, Human, aa1-173, His-tag (Myosin Light Chain 5, Myosin Regulatory Light
Chain 5, Superfast Myosin Regulatory Light Chain 2, MYLC2, MyLC-2)
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| MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. | | | Catalog # | 029673 | | Source | Recombinant corresponding to aa1-173 from human MYL5, fused to His-tag at N-terminal expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL | | DYKALSYVIT HGEEKEE | | Molecular Weight | ~22.1kD (MALDI-TOF) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~22.1kD | | Source | E.coli | | Purity | ~90% (SDS-PAGE) | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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