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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomiclysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
Catalog #	029725
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa226 from rat Apg5.
Specificity	Recognizes rat Apg5/Atg5 at ~37kD. Species Crossreactivity: human, mouse, porcine and bovine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.