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You are here:Home » Antibodies » Abs to Autophagy » Anti -Atg5 (Autophagy Protein 5)

Anti -Atg5 (Autophagy Protein 5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomiclysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
Catalog #029725
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa226 from rat Apg5.
SpecificityRecognizes rat Apg5/Atg5 at ~37kD. Species Crossreactivity: human, mouse, porcine and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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