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Specifications

Clone Host Grade Applications Monoclonal Mouse E B IH

Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Catalog #	030131
Applications	Western Bloting: 1:500-1:2000.
	Immunohistochemistry: 1:200-1:1000.
	Immunofluorescence: 1:200-1:1000.
	ELISA: Suggested dilution 1:10000.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	13B209
Host	Mouse
Source	Human
Concentration	As reported
Form	Ascites fluid, 0.03% sodium azide.
Purity	Ascites
Immunogen	Purified recombinant fragment of CK5 expressed in E. coli.
Specificity	Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	K5; DDD; EBS2; KRT5A; KRT5