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You are here:Home » Antibodies » Abs to Dickkopf Proteins » Anti -DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)

Anti -DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IH FC
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Catalog #030149
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50-55aa in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12aa. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.
ApplicationsWestern Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Flow Cytometry: 1:200-1:400
ELISA: 1:10000
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No13B227
HostMouse
SourceHuman
ConcentrationAs reported
FormPurified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
PurityPurified
ImmunogenPurified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. coli.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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