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Specifications

Clone Host Grade Applications Monoclonal Mouse E B

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.
Catalog #	030281
Applications	Western Bloting: 1:500-1:2000.
	ELISA: Suggested dilution 1:10000.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	13B359
Host	Mouse
Concentration	As reported
Form	Purified antibody in PBS, 0.03% sodium azide.
Purity	Purified
Immunogen	Purified fusion protein with GST protein tag expressed in E. coli.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.