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Specifications

Clone Host Grade Applications Monoclonal Mouse E B

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NF B is retained in the cytoplasm in an inactive form by the inhibitory protein I B. Activation of NF B requires that I B be phosphorylated on specific serine residues, which results in targeted degra-dation of I B. I B kinase (IKK ), previously designated CHUK, interacts with I B- and specifically phosphorylates I B- on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKK , IKK and IKK (also designated NEMO), and each appear to make essential contributions to I B phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKK and IKK . IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNF , IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of I B on Ser32 and Ser36, and in NF B activation, suggesting that IKK-i may act as an I B kinase in the immune system.
Catalog #	030329
Applications	Western Bloting: 1:500-1:2000.
	ELISA: Suggested dilution 1:10000.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	13B407
Host	Mouse
Source	Human
Concentration	As reported
Form	Ascites fluid, 0.03% sodium azide.
Purity	Ascites
Immunogen	Purified recombinant fragment of IKBKE (aa1-257) expressed in E. coli.
Specificity	Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	IKBKE