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030478 Mouse Anti-NCK1

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P16333
Isotype
IgG1
Clone Number
13B556
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu Mk
Gene ID
4690
Shipping Temp
Blue Ice
Storage Temp
-20°C
NCK; nck-1; NCKalpha

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human NCK1 expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified
Specificity
Human, Monkey
References
1. Mol Cell Biol. 2008 Mar;28(6):2035-46. |2. Cell Signal. 2010 May;22(5):848-56.|
USBio References
No references available
Conjugates
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