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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S-Transferase (GST)

Anti -Glutathione S-Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Catalog #031005
ApplicationsSuitable for use in Western Blot, ELISA.
Recommended DilutionELISA: 1:3,000
Western Blot: 1:2,000
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9AT106
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant GST fusion protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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