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You are here:Home » Antibodies » Abs to Infectious Disease Neisseria » Anti -Mobc (Mobilization protein C, mobc) (AP)

Anti -Mobc (Mobilization protein C, mobc) (AP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The mobilization region of the 7.4 kb beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae was characterized. The 3.2 kb HindIII-BamHI fragment of pSJ7.4 was mobilized between Escherichia coli strains by conjugative plasmid RK2. Selected restriction enzyme-generated deletions of this fragment were subcloned in pACYC177 to obtain constructs that were suitable for analysis of the mobilization region. Mating experiments showed that a highly conserved 1.9 kb DNA region within coordinates 4096-5997 is required for mobilization of pSJ7.4. This region contains two genes encoding the mobilization protein MobA, another protein, MobC, putatively involved in mobilization from some hosts, and an intergenic oriT. The 168 bp intergenic sequence also contains the promoters for mobA and mobC in an arrangement that suggests divergent transcription and autoregulation from oriT. The 56 kDa MobA was expressed in E. coli as a (6x)His-Tag fusion protein. Purified MobA specifically induced plasmid relaxation by nicking at the oriT. MobA is exceptional because the N-terminal region alone can mobilize pSJ7.4, albeit at a lower frequency than the full-length protein, even in the absence of MobC. The carboxyl terminal region of MobA did not share homology with other mobilization proteins, but may be involved in promoting efficient transfer of pSJ7.4.
Catalog #031133-AP
ApplicationsSuitable for use in Western Blot, ELISA.
Recommended DilutionELISA: 1:1000
Western Blot: 1:100-500
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C with the addition of glycerol. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
IsotypeIgG2a
Clone NoID:19CT.2.8.14
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.
PurityPurified by Protein G affinity chromatography.
ImmunogenMobc antibody was raised using purified His-tagged recombinant full length Neisseria gonorrhoeae plasmid pSJ7.4 mobilization protein C (mobC)and mobilization protein A (mobA) genes.
SpecificityNeisseria gonorrhoeae
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMobilization protein C, mobc


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