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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12)

Anti -GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase
GAPDH, GAPD, CDABP0047, OK/SW-cl.12)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC IF
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). The HGNC name for this protein is GAPDH.
Catalog #031366
ApplicationsSuitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunocytochemistry (ABC): 1:5000
Immunofluorescence: 1:500-1:1000
Western Blot: 1:20,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourcePorcine
ConcentrationNot determined
FormSupplied as a liquid.
ImmunogenExtensively purified porcine GAPDH
SpecificityRecognizes GAPDH from human, bovine, porcine, mouse, rat and other mammals, and also recognizes avian GAPDH. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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