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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Lamin A, C (Lamin A/C, Lamin-A/C, LMNA, LMNC, 70kD Lamin, LMN1, LMNL1, Renal Carcinoma Antigen NY-REN-32)

Anti -Lamin A, C (Lamin A/C, Lamin-A/C, LMNA, LMNC, 70kD Lamin, LMN1, LMNL1, Renal
Carcinoma Antigen NY-REN-32)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IF
The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74kD while Lamin C is 65kD. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. This is the case with this polyclonal, which was raised against full length recombinant human Lamin A. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3). The HGNC name for this protein is LMNA.
Catalog #031382
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:5000
Western Blot: 1:5000 using chemiluminescence.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium azide.
PuritySerum
ImmunogenRecombinant full length purified Lamin A from E. coli.
SpecificityRecognizes Lamin A and Lamin C from human, bovine, porcine, mouse, rat and other mammals. Since Lamin A/C is highly conserved, it is likely that the antibody is effective on other species also.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, PRO1


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