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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -MARCKS (Myristoylated Alanine-rich C-kinase Substrate, MACS, 80K-L Protein, Protein Kinase C Substrate, 80 kD Protein Light Chain, PKCSL, PRKCSL)

Anti -MARCKS (Myristoylated Alanine-rich C-kinase Substrate, MACS, 80K-L Protein, Protein
Kinase C Substrate, 80 kD Protein Light Chain, PKCSL, PRKCSL)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC IF
Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epitethelial cells (1). The MARCKS protein runs at about 80kD on SDS-PAGE gels, although the real molecular is much lower, at about 31.5kD. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (2). MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties (3). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (4). The HGNC name for this protein is MARCKS.
Catalog #031386
ApplicationsSuitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunocytochemistry (ABC): 1:5000
Immunofluorescence: 1:500-1:1000
Western Blot: 1:20,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid.
PuritySerum
ImmunogenFull length recombinant MARCKS expressed in and purified from E. coli.
SpecificityRecognizes rat MARCKS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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