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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Microtubule Associated Protein 2 (MAP2, MAP-2)

Anti -Microtubule Associated Protein 2 (MAP2, MAP-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (2). MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kD on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. The HGNC name for this protein is MAP2.
Catalog #031390
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:1000
Western Blot: 1:10,000 using chemiluminescence.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No13B833
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 10mM sodium azide.
PurityPurified by affinity chromatography.
SpecificityRecognizes MAP2 from rat, mouse, human and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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